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The active-site cavity, which is located between the two domains, comprises a glutathione binding site (G-site), formed mainly by the N-terminal domain, and a hydrophobic substrate-binding site (H-site), which is formed by the C-terminal domain
Then, 20 L of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 l of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone
GHK-Cu: copper transport, ECM remodeling, redox modulation GHK-Cu is a coordination complex between the tripeptide Gly-His-Lys and Cu(II), first isolated from human plasma by Loren Pickart in 1973
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